This study assessedknowledge and practice of cholera prevention strategies among junior secondary school students in Katsina State, Nigeria. Ex-post facto research design was used. The respondents used for the purpose of data collection were four hundred (400) sampled from a population of 271,690 junior secondary school students inKatsina State Nigeria. The respondents were drawn through multistage sampling technique, which consisted of stratified, simple random sampling andproportionate sampling techniques. A close ended questionnaire was used to obtain responses from the participants. Three hundred and ninety-eight (398; 99.5%) questionnaires were returned. Descriptive statistics of frequency, percentages, means and standard deviations were used to describe the demographic characteristics of the respondents.The hypotheses were tested at 0.05 level of significance using one sample t-test, independent sample ttest and analysis of variance. The findings of the study revealed that knowledge of cholera prevention strategies among junior secondary school students in Katsina State of Nigeria was significantly adequate (t = 3.070; p= 0.001), practice of cholera prevention strategies among junior secondary school students in Katsina state of Nigeria was not significant (t=1.291; p= 0.110), junior secondary school students in Katsina State of Nigeria do not significantly differ in their knowledge of cholera prevention strategies based on demographic characteristics(p = 0.17, 0.21, 0.10)and junior secondary school students in Katsina State, Nigeria do not significantly differ in their practices of cholera prevention strategies based on demographic characteristics (0.7, 0.5, 0.1). On the basis of the findings of the study, it was concluded that Junior secondary school students in Katsina State of Nigeria have knowledge of cholera prevention strategies, but they do not practicecholera prevention strategies,and they do notdiffer in their knowledge and practice of cholera prevention strategies based on demographic characteristics. However, It was,recommended that: school managements should put in place adequate measures within the school environment to checkmate and control the healthy practices of student this include provision of hand washing sink, provision of safe drinking water, supervising the canting, provision of charts and notice board within the school environment and adequate use of latrine by students.